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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 21, No. 2, 2018, pp. 169-174
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Bioline Code: md18027
Full paper language: English
Document type: Research Article
Document available free of charge
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African Journal of Biomedical Research, Vol. 21, No. 2, 2018, pp. 169-174
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Plasma and Aqueous Humour Electrolyte Levels, Sodium Pump Activity and Intraoccular Pressure in Glaucoma and Cataract Subjects attending a Nigerian Tertiary Hospital
Omotosho, I.O; Nwachukwu, C.N; Olawoye, O.O; Oluleye, T.S & Ayeotan, T
Abstract
In this case-controlled study, electrolyte levels in Aqueous Humour (AH) and plasma, sodium/potassium adenosine
triphosphatase (Na+/ K+ ATPase) activity as well as intra-occular pressure were measured in normal, cataract and glaucoma
participants as possible biomarkers of degenerative diseases of the eye. Three groups of age-matched consenting participants
were recruited viz: Non-visually impaired Control, participants with Open-angle glaucoma (cases) and those with cataract
(Negative control). AH fluid was surgically collected from glaucoma and cataract participants. Sodium, potassium, chloride and
bicarbonate levels were determined in plasma and in the AH fluid using ISE-4000. Red-cell membrane Na+/ K+ ATPase activity
and plasma glucose were assayed spectrophotometrically. Blood pressure (BP) and pH were monitored in all participants using
standard methods. Data were analysed using ANOVA and significance was at p<0.05. All participants were normotensive and
normoglycaemic. Mean Intra-occular Pressure (IOP) was significantly higher in glaucoma. Mean plasma and AH Na+, K+ and
Cl- levels in the three groups of participants were similar. Mean AH Cl- (121.70±5.4mmol/L) and HCO3- (13.55 ± 3.71mmol/L)
levels in glaucoma were significantly higher when compared with cataract and control groups. Mean Na+/K+ ATPase activity
in cases was low (22.35 ±1.09µmiP/µgP/min) in comparison to that of cataract and control [23.40±1.09 and 25±2.0µmiP/µgP/min
respectively]. The mean pH of AH in cases was 6.7 in comparison to a pH of 7.2 in all participants plasma. Increased levels of
Cl- and HCO3- ions were observed in AH in glaucoma and this may be prognostic for glaucoma subjects.
Keywords
glaucoma; electrolyte levels; Na/K ATPase activity; anion/cation imbalance
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© Copyright 2018 - African Journal of Biomedical Research
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