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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 16, No. 1, 2017, pp. 161-164
Bioline Code: pr17021
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 1, 2017, pp. 161-164

 en Identification of active anti-inflammatory principles of beta-beta wood ( Lunasia amara check for this species in other resources Blanco) from Siawung Barru-South Sulawesi, Indonesia
Hasnaeni; Sudarsono; Nurrochmad, Arief & Widyarini, Sitarina


Purpose: To identify the anti-inflammatory components of beta-beta (Lunasia amara Blanco.) wood.
Methods: The wood material was extracted with 96 % ethanol and fractionated with dichloromethane using a liquid-liquid continuous extraction (LLCE). The fractions were subjected to silica gel column chromatography. Components of the extracts were identified by thin layer chromatography (TLC) scanner and UV-visible spectroscopy, using scopoletin as standard.
Results: TLC results for Lunasia amara extract showed the same spot as standard scopoletin. UV-visible spectrum for scopoletin displayed maximum absorption at 213, 228, 255 and 344 nm, while betabeta wood extract showed characteristic bands at 344, 336, 299 and 255 nm. The results indicate that the main components of the extracts are scopoletin and its derivatives.
Conclusion: The active anti-inflammatory compound in beta-beta (Lunasia amara) wood is scopoletin.

Beta-beta wood (Lunasia amara Blanco.); Scopoletin; Thin layer chromatography

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