About Bioline  All Journals  Testimonials  Membership  News

Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 16, No. 6, 2017, pp. 1317-1323
Bioline Code: pr17168
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 16, No. 6, 2017, pp. 1317-1323

 en Mesenchymal stem cells promote incision wound repair in a mouse model
Li, Dong-Jie; Shen, Chuan-An; Sun, Tian-Jun; Zhang, Lin; Deng, Hu-Ping & Chai, Jia-Ke


Purpose: To investigate the wound healing process via the application of mesenchymal stem cells (MSCs) in a mouse model.
Methods: MSCs were collected from the bone marrow of the femur and tibia of 6 – 12-week-old C57BL/6 mice. Full-thickness cutaneous wounds (4 × 2 cm) were made by incision on the dorsal side of the mice. The wound was then subjected to one of four random treatments: phosphate-buffered saline (PBS) solution, 3T3 fibroblasts, naive MSCs, or interferon gamma-activated MSCs. Chalkley method was used to determine vascular density. A score was given, for each field examined, for CD31-positive areas, and the results of blind analysis were confirmed by independent analysis of a second evaluator.
Results: The tensile strength of the wound area was significantly lower in older versus younger mice (p ≤ 0.0007). Only one quarter of the mean force was required to disrupt wound integrity in older mice compared to young mice. Treatment with MSCs showed positive effects on wound healing. Activated MSCs showed the greatest efficacy at a dosage of 5 × 104 activated MSCs/8 cm2 of wound area or 6, 250 cells/cm2.
Conclusion: The results suggest that MSC therapies enhance the tissue regeneration capacity in mice, especially in older populations, through effective transdifferentiation into the epithelium.

Mesenchymal stem cell; wound healing; mouse

© Copyright 2017 - Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil