BIOTECH RESEARCH - A MODIFIED AND SIMPLE METHOD FOR THE EXTRACTION OF GENOMIC DNA FROM A WIDE VARIETY OF ANIMAL AND PLANT TISSUES|
Diana Sara Leal-Klevezas, Juan Pablo Martínez-Soriano
Every DNA extraction procedure must break the cell by disrupting membranes and cell walls, inactivate DNAses and remove other macromolecules. Many techniques have been described to isolate DNA from specific types of cells, but they do not necessarily apply to different types of cells. Here we are reporting a DNA extraction method that yields DNA in good amounts and purity to perform PCR, RAPD, AFLP, restriction analysis and cloning. This protocol has proved to be useful not only from small amounts of samples from a wide variety of organisms and tissues alone, but also in complex cell mixtures and food products, by reducing the presence of residual inhibitors in the reactions. The described method not only is very versatile, saves time and avoids using fridge and bench space to store endless DNA extraction solutions, but is also simple, inexpensive and yields DNA that is stable for years.
DNA extraction, animal, insect, fungi, virus, dairy products