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Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 13, No. 2, 1996, pp. 81-88
Bioline Code: ba96036
Full paper language: Spanish
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 13, No. 2, 1996, pp. 81-88

 en
Pardo, Orlando L.

Abstract

In the last five years, it has been found that direct nucleic acid transfer into living organisms is a feasible approach to obtain expression levels that although are still far from being of any therapeutic value, are indeed enough to elicit in the host a significative immune response against the protein codified by the nucleic acid as such. This methodology of immunization has many advantages over more conventional ones, namely: the need of purifying proteins is obviated, as the expression occurs in the host cells the appropriate protein processing and conformation are guaranteed, and most pros of live (e.g. elicitation of an efficient cellular response) and subunit vaccines (e.g. high safety) are combined while most cons of both are avoided (e.g. elicitation of an immune response against the proteins of the specific live vector used). Therefore, although many questions on the topic still remain unanswered, the possibility of nucleic acid vaccines becoming a new generation of human vaccines belongs now to the near future.

Keywords
DNA immunization, DNA vaccines, gene-transfer, plasmid DNA

 
 es La inmunizacion con ADN: Una nueva generacion de vacunas?
Pardo, Orlando L.

Resumen

En los ultimos cinco anos se ha logrado demostrar que la transferencia directa de acidos nucleicos a organismos vivos genera niveles de expresion que si bien son aun de escaso valor terapeutico, son suficientes para despertar en el hospedero una respuesta inmune significativa contra la proteina codificada por el acido nucleico en cuestion. Esta metodologia de inmunizacion posee multiples ventajas sobre las ya convencionales: se obvia la necesidad de purificar proteinas, la expresion en las propias celulas del hospedero garantiza una maduracion y conformacion adecuadas para la proteina de interes, y se combinan muchos aspectos positivos de las vacunas vivas (p.e. obtencion de respuesta celular eficiente) con los de las vacunas de subunidades (p.e. alta seguridad), evitandose ademas muchos de los aspectos negativos de ambas (p.e. desencadenamiento de respuesta contra los antigenos propios del vector vivo empleado). Por consiguiente, y aunque aun existen muchas interrogantes abiertas al respecto, la posibilidad de que las vacunas de acidos nucleicos se conviertan en una nueva generacion de vacunas humanas pertenece ahora a un futuro no muy lejano.

Palabras-clave
ADN plasmidico, transferencia genetica, vacunas de ADN

 
© Copyright 1996 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

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