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Berlanga, Jorge; Moreira, Evelio; Perez, Luis C.; Boix, Eduardo; Gonzalez, Tania & Lopez-Saura, Pedro Resumen Actualmente no se dispone de elementos experimentales, que avalen la utilizacion de determinadas dosis de Factor de Crecimiento Epidermico (EGF) en formulaciones semisolidas topicas para estimular la cicatrizacion. Para realizar esto, se utilizaron grupos de 18 ratas hembras Sprague Dawley lesionadas experimentalmente, asignadas a: A - grupo no tratado o tratado con B, B - vehiculo hidrofilo, C - 0,5 ug de EGF humano recombinante (rh-EGF)/g; D - 5 ug rh-EGF/g y E - 10 ug rh-EGF/g de vehiculo. Al septimo tratamiento, las lesiones fueron resecadas y procesadas para evaluar los siguientes aspectos: reepitelizacion, porciento de animales por grupo con lesiones epitelizadas a mas de 90 %, espacio no epitelizado y nivel de contraccion de la herida. Tambien se estudio la relacion entre dosis, la intensidad del infiltrado inflamatorio y la respuesta fibrovascular en cada grupo. La reepitelizacion fue significativamente superior en los grupos D y E comparados con los grupos A, B y C. El 47 % de los animales en el grupo E mostro mas del 90 % de reepitelizacion comparado con los grupos A, B y C (p < 0,05). Los grupos D y E mostraron respuestas similares en cuanto a contraccion de herida y espacio reepitelizado entre los bordes. La respuesta inflamatoria en los grupos D y E fue significativamente menor y se constato una respuesta fibroplastica superior a los grupos A, B y C. Palabras-clave reepitelizacion, contraccion de herida

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Wound healing promotion in rats treated with EGF is dose dependent Berlanga, Jorge; Moreira, Evelio; Perez, Luis C.; Boix, Eduardo; Gonzalez, Tania & Lopez-Saura, Pedro Abstract The relationship between Epidermal Growth Factor (EGF) dose and healing activity, has not been established when EGF is formulated as a semisolid cream. In order to address this question, groups of 18 experimentaly wounded Sprague Dawley rats were assigned to: A - untreated or treated with B, B - hydrophilic vehicle only, C - 0.5 ug recombinant human EGF (rh-EGF)/g; D - 5 ug rh-EGF/g and E - 10 ug rh-EGF/g of hydrophilic vehicle using a full-thickness skin wound model. After seven days, re-epithelization, percent of animals per group with re-epithelized lesions to more than 90 %, non-epithelized space between edges, and wound contraction level were assayed. The relationship between doses, inflammatory infiltrate and the fibrovascular reaction were also evaluated. Re-epithelization was significantly enhanced in groups D and E compared to A, B and C. Fourty seven percent of animals in group E showed re-epitelization of lessions to more than 90 % compared to groups A, B and C (p < 0.05). With respect to wound contraction and the amounts of non re-epithelized space between edges, groups D and E exhibited similar responses, which were significantly improved with respect to groups A, B or C. Inflammatory infiltrate was significantly reduced in groups D and E, and showed a highest fibrovascular reaction compared to groups A, B and C. Keywords re-epithelization, wound contraction

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