search
for
 About Bioline  All Journals  Testimonials  Membership  News


Biotecnologia Aplicada
Elfos Scientiae
ISSN: 0684-4551
Vol. 14, No. 3, 1997, pp. 169-174
Bioline Code: ba97039
Full paper language: English
Document type: Research Article
Document available free of charge

Biotecnologia Aplicada, Vol. 14, No. 3, 1997, pp. 169-174

 en Genetic transformation of sugarcane by Agrobacterium tumefaciens check for this species in other resources using antioxidant compounds
Enriquez-Obregon, Gil A.; Vazquez-Padron, Roberto I.; Prieto-Samsonov, Dmitri L.; Perez, Marlene & Selman-Housein, Guillermo

Abstract

The effects of 3 antioxidant compounds -ascorbic acid, cysteine and silver nitrate- on the growth of the Agrobacterium tumefaciens check for this species in other resources strain At 2260 and its interaction with plant cells, as well as on the viability and callus formation of meristematic tissue, were evaluated using explants from in vitro and field-cultured sugarcane plants commercial variety Ja 60-5. We achieved the transfer of a binary vector bearing the uid1 and bar genes to sugarcane meristematic explants, and obtained the generation of BASTAR-resistant and GUS-positive calli. The use of an antioxidant mix caused an 80 % cell death decrease in respect to controls, and the callus quality was not affected in any of the culture phases.

Keywords
transgenic plants, plant-microorganism interaction, hypersensitivity, necrogenesis, BASTAR, GUS

 
 es
Enriquez-Obregon, Gil A.; Vazquez-Padron, Roberto I.; Prieto-Samsonov, Dmitri L.; Perez, Marlene & Selman-Housein, Guillermo

Resumen

Se evaluaron los efectos de tres compuestos antioxidantes - acido ascorbico, cisteina y nitrato de plata - sobre el crecimiento de Agrobacterium tumefaciens check for this species in other resources cepa At 2260 y la interaccion de este con los explantes vegetales, asi como sobre la viabilidad y la formacion de callos en tejidos meristematicos de cana de azucar variedad comercial Ja 60-5, provenientes de plantas in vitro y de campo. Se logro la transferencia de un vector binario con los genes uid1 y bar a explantes meristematicos de cana de azucar y se obtuvieron callos resistentes a BASTAR y GUS-positivos. El empleo de la mezcla de antioxidantes redujo en un 80 % la muerte celular con relacion al control, y no altero la calidad del callo en ninguna de las fases de cultivo.

Palabras-clave
plantas transgenicas, interaccion planta-microorganismo, hipersensibilidad, necrogenesis, BASTAR, GUS

 
© Copyright 1997 Elfos Scientiae
Alternative site location: http://elfosscientiae.cigb.edu.cu/Archivo.asp?Id=6

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil