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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 13, No. 1, 2003, pp. 23-30
Bioline Code: bk03004
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 13, No. 1, 2003, pp. 23-30

 en Synergistic Interaction Between Two Linear Inhibitors On A Single Enzyme: Vanadate And L-phenylalanine Inhibition Of Rat Liver Alkaline Phosphatase
Malomo, Sylvia O.; Olorunniji, Femi J.; Arise, Rotimi O.; Adebayo, Joseph O.; Adedosu, Olaniyi T. & Odutuga, Adewale A.

Abstract

The combined effect of two linear inhibitors of rat liver alkaline phosphatase (ALP). vanadate (Van) and L-phenylalanine (L-phe) were studied using a modification of the common Yonetani-Theorell procedure proposed for studying synergistic inhibition. The modes of inhibition of ALP by Van and L-phe as analysed using the double reciprocal plots of the Michaelis-Menten equation were mixed and uncompetitive inhibition respectively. Analysis of the combined effect of the two inhibitors showed that their inhibitory effects were mutually enhanced. The mechanistic aspects and practical applications of the procedure are discussed.

Keywords
alkaline phosphatase, synergistic inhibition

 
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