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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 15, No. 2, 2003, pp. 84-92
Bioline Code: bk03024
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 15, No. 2, 2003, pp. 84-92

 en Effect of Stem Bark Extracts of Enantia Chloranta check for this species in other resources On Some Clinical Isolates
Atata, Razaq F.; Sani, Alhassan & Ajewole, Stella M.

Abstract

Studies on antimicrobial activity of water, methanol and ethanol extracts of the stem bark of Enantia chloranta check for this species in other resources , was carried out by the agar ditch diffusion and tube dilution methods. The clinical isolates used include; Staphylococcus aureus check for this species in other resources , Streptococcus pyogenes check for this species in other resources , Shigella sonnei check for this species in other resources , Proteus mirabilis check for this species in other resources , Klebsiella pneumoniae check for this species in other resources , Salmonella typhi check for this species in other resources , Pseudomonas aeruginosa check for this species in other resources , Aspergillus niger check for this species in other resources , and Candida albicans check for this species in other resources . The isolates were obtained from the Department of Medical microbiology and Parasitology of the University of Ilorin Teaching Hospital. Crude extracts of all solvents used inhibited the growth of all the isolates tested, except Pseudomonas aeruginosa . The Minimum Inhibitory Concentration (MIC) ranged between 25mg/ml to 150mg/ml depending on isolate and extracting solvent. Ethanolic extracts showed greater antimicrobial activity than the other two solvents. The killing rate of the Minimum Bactericidal Concentration (MBC) of the ethanolic extract on S. aureus and P.aeruginosa was 21/2;hr, and 3hr respectively. Temperature stability study showed that the extracts are stable and active over a temperature range of 30 to 121°C.

Keywords
Enantia chlorantha, antimicrobial property, plant extract

 
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