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Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 18, No. 1, 2006, pp. 56-61
Bioline Code: bk06009
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 18, No. 1, 2006, pp. 56-61

 en Behavior Of Manganese Ion In Basic Medium: Consequence For The Measurement Of Enzymatic Activities Using Nitrophenyl Derivatives
Niamke, Sébastien; Kouame, Lucien Patrice; Due, Edmond Ahipoh & Dabonne, Soumaila

Abstract

The use of nitrophenyl derivatives in the determination of the influence of manganese ion on catalytic activities of glycosidases and phosphatases remains a problem. In fact, in this study, adding manganese chloride or manganese sulfate to sodium hydroxyde or sodium carbonate in aqueous solution yielded secondary reactions. These secondary reactions affected the absorbance for wavelengths varying between 400-420 nm. This chemical behavior did not allow reliable results in determining the influence of manganese ion on glycosidase and phosphatase activities, when nitrophenyl derivatives were used as substrates. This study showed that this problem could be avoided when natural substrates such as disaccharides (glycosidases) and phosphorylglycosides (phosphatases) were used in enzymatic tests.

Keywords
phosphatase, glycosidase, manganese ion, nitrophenyl derivatives, basic medium, enzymatic determination

 
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