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Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 18, No. 2, 2006, pp. 63-69
Bioline Code: bk06010
Full paper language: English
Document type: Research Article
Document available free of charge

Biokemistri, Vol. 18, No. 2, 2006, pp. 63-69

 en The Effects Of Ascorbic Acid On Salt Induced Alfalfa ( Medicago Sativa check for this species in other resources L.) In In Vitro Culture
Arab, Lila & Ehsanpour, Ali Akbar


Ascorbic acid as an antioxidant agent has already been used for increasing of stress tolerance. Callus was produced from stem segments of alfalfa ( Medicago sativa check for this species in other resources L.) on MS medium supplemented with 2,4 dichlorophenoxy acetic acid, naphthalene acetic acid and kinetin (2 mg/l each).Calli were then transferred to the same medium containing 0, 30, 60, 90, 120 mM NaCl and 0, 0.5, 1.0, 2.0 mM ascorbic acid. Addition of ascorbic acid to the medium improved seed germination and also increased the activity of acid phosphates, chlorophyll content, and dry mass. The Na+ and K+ content of stem-leaf and root was relatively increased with some variations. The fresh weight of calli was also increased by ascorbic acid under salt stress condition

Ascorbic acid, in vitro, Medicago sativa, salt stress

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