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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 69, No. 1, 2009, pp. 112-118
Bioline Code: cj09014
Full paper language: Spanish
Document type: Note
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 69, No. 1, 2009, pp. 112-118

 en ENCAPSULATED SOMATIC EMBRYOS AND ZYGOTIC EMBRYOS FOR OBTAINING ARTIFICIAL SEEDS OF RAULI-BEECH ( Nothofagus alpina check for this species in other resources (Poepp. & Endl.) Oerst.)
Cartes R., Priscila; Castellanos B., Hermes; Ríos L., Darcy; Sáez C., Katia; Spierccolli H., Scarlette & Sánchez O., Manuel

Abstract

Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were evaluated after 4 wk. Comparing the percentages of germination reached by encapsulated somatic and zygotic embryos it was observed that they had similar germinative behavior according to the type of encapsulation applied. However, zygotic embryos substantially exceeded the germination levels reached by somatic embryos, 100% vs. 45% respectively.

Keywords
rauli-beech, somatic embryogenesis, synthetic seed, sodium alginate.

 
 es Encapsulado de embriones somáticos y embriones cigóticos para obtención de semillas artificiales de raulí ( Nothofagus alpina check for this species in other resources (Poepp. & Endl.) Oerst.).
Cartes R., Priscila; Castellanos B., Hermes; Ríos L., Darcy; Sáez C., Katia; Spierccolli H., Scarlette & Sánchez O., Manuel

Resumen

Embriones somáticos y cigóticos provenientes de semillas maduras de raulí, Nothofagus alpina (Poepp. & Endl.) Oerst., se encapsularon en diferentes endospermas sintéticos con el fin de generar una cubierta que cumpla la función de nutrir y proteger al embrión para facilitar su posterior germinación. Se varió el contenido de alginato de sodio al 4%, 3% y 2% y el tiempo de inmersión en cloruro de calcio (CaCl2), el que actúa como agente acomplejante. Además, a la matriz artificial se adicionaron componentes del medio Murashige y Skoog (MS) suplementado con: 0,5 mg L-1 de indolacetic acid (IAA), 0,5 mg L-1 de ácido naftalenacético (NAA), 2 mg L-1 de 6-bencilaminopurina (BAP) y 30 g L-1 de sacarosa. Al cabo de 4 semanas el porcentaje de germinación de los embriones somáticos y cigóticos encapsulados tuvieron similar comportamiento germinativo según el tipo de encapsulado aplicado. Sin embargo, los embriones cigóticos superaron ampliamente los niveles de germinación alcanzados por los embriones somáticos, 100% vs. 45%, respectivamente.

Palabras-clave
raulí, embriogénesis somática, semilla sintética, alginato de sodio.

 
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