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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 11, No. 2, 2003, pp. 97-106
Bioline Code: cs03012
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 11, No. 2, 2003, pp. 97-106

 en Variation Of β-1,3-Glucanas, Chitnase And Polyphenoloxidase Activities In Cacao Pods Upon Phytophthora Megakarya check for this species in other resources Inoculation
Omokolo, N.D.; Nankeu, D.J.; Niemenak, N. & Boudjeko, T.

Abstract

Cacao ( Theobroma cacao check for this species in other resources L.) clones that differ in susceptibility to black pod disease were analysed for response to stress induced by pod inoculation with the fungus Phytophthora megakarya check for this species in other resources Braz. Et Griff. Fungal inoculation significantly stimulated β-1,3-glucanase activity in both soluble and ionically-bound fractions of the less susceptible clones. No significant correlation was found in the other clones. Chitinase activity in both soluble and ionically-bound fractions was generally reduced upon pod inoculation with the fungus, but this pattern did not correlate with disease severity. Polyphenoloxidase activity, following pod inoculation, was genotype dependent in the soluble fractions, whereas in the ionically-bound fractions, stimulation of this activity was recorded in the less susceptible clones. Moreover, pod inoculation was characterised by the appearance of a new polyphenoloxidase isoform A'2 in the less susceptible clones.

Keywords
Absorbance, cortex, ionically-bound, Theobroma cacao check for this species in other resources , wonding

 
 fr
Omokolo, N.D.; Nankeu, D.J.; Niemenak, N. & Boudjeko, T.

Résumé

Les réponses aux stress induits par innoculation de gousses de cacao ( Theobroma cacao check for this species in other resources L.), aux susceptibilités differentes à la maladie de Blackpod, par le Phytophthora megakarya check for this species in other resources Braz étaient analysées. L'inoculation de fungi a significativement stimulé les activités de « glucanase » β-1,3 dans les deux fractions solubles et à liaison ionique des clones moins susceptibles. Pas des corrélations significatives étaient observées dans d'autres clones. L'activité de la chitanase dans les deux milieux était generalement reduite juste après l'inoculation par le fongi, mais cette tendance n'a pas corrélée avec la sévérité de la maladie. L'activité du polyphenoloxidase, après inoculation de gousses, était généralement dependante du génotype dans la fraction soluble, alors que la stimulation de cette activité était observée dans les clones moins susceptibles. En plus, l'inoculation des gousses était caracterisée par l'apparition de nouveau polyphenolosidase isoformes A'2 dans les clones les moins susceptibles.

 
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