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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 4, No. 1, 1996, pp. 89-95
Bioline Code: cs96044
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 4, No. 1, 1996, pp. 89-95

 en Some Characteristics of Pea Necrosis Elicitor from Compatible Interactions of Pseudomonas Syringae p.v. Pisi and Pisum Sativum
Mwang'ombe, A.W. & Archer, S.A.


Specific elicitors of pea necrosis were obtained from intercellular fluids of compatible race-cultivar interactions of Pseudomonas syringae pv. pisi and pea (Pisum sativum ). These elicitors induced necrosis in resistant but not in susceptible cultivars. Using pea cultivars carrying different resistance genes to the bacterial races 1 to 5 as test plants, an elicitor of necrosis was isolated and partially characterised. The necrosis inducing activity of these intercellular fluids was not lost after dialysis, gel-filtration and isoelectric focusing. The dialysed and lyophilised elicitor-active fraction of the intercellular fluids contained considerable amounts of protein and carbohydrate, and was partially sensitive to subtilisin BPN, proteinase K and protease V, but not to trypsin. These elicitors were inactivated by heat at 60 C and also by periodate. Most of the elicitor activity was recovered in a fraction of pH 11.7 after isoelectric focusing, suggesting a highly basic nature.

Carbohydrate, dialysis, gel filtration, isoelectric focusing, protein

Mwang'ombe, A.W. & Archer, S.A.


Des inducteurs specifiques de necrose ont ete obtenus des fluides intercellulaires d 'interactions compatibles de races de cultivars du Pseudomonas syringae pv.pisi et pois (Pisum sativum). Ces inducteurs declanchent la necrose dans les cultivars resistants mais pas dans ceux qui sont suceptibles. En utilisant comme plantes test des cultivars de pois ayant de genes de resistance aux races bateriennes 1 a 5, un inducteur de necrose a ete isole et partiellement caracterise. Les activites declanchees par la necrose dans les fluides intercellulaires n'ont pas ete perdues apres la dialyse, la filtration colloidale et la fixation isoelectrique. La dialyse et la fraction active lyophilisee et dialysee des inducteurs des fluides intercellulaires contenaient des quantites considerables de proteines et d'hydrates de carbone, et etaient partiellement sensible a la subtilisine BPN, a la proteinase K eta la protease V mais pas a la trypsine. Ces inducteurs etaient inactives par la chaleur a 60 C ainsi que par le periodate. La plupart des activivites des inducteurs ont ete recuperees dans une fraction de pH 11.7.

Mots Clés
Carbohydrates, dialyse, infiltration de gel, ciblage isoelectrique, proteine

© Copyright 1996 - African Crop Science Society

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