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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 5, No. 3, 1997, pp. 239-251
Bioline Code: cs97030
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 5, No. 3, 1997, pp. 239-251

 en In vitro shoot multiplication of Eucalyptus grandis
Wachira, F.

Abstract

In vitro shoot multiplication was induced from sections of orthotropic shoot sprout regrowth of 25 year old pruned Eucalyptus grandis using a regeneration medium supplemented with several levels of different plant growth regulator combinations, different carbon sources, and different pH at time of inoculation. Optimal shoot regeneration was obtained in Murashige and Skoog medium supplement with 0.4mg.l-1 BAP and 1.0mg.l-1 IBA or 0.2mg.l-1 BAP and 1.0mg.l-1 NAA and 3% (w/v) sucrose. An 8-week subculture period was found to be suitable. Both NAA and IBA decreased the shelf life of E. grandis shoot multiplication cultures by encouraging callus growth and proliferation. Sucrose at 30g.l-1 gave superior growth compared to the other three carbon sources tested, i.e., maltose, glucose and fructose. The pH of the medium affected growth of the shoot cultures with a pH of 5.8 giving better growth.

Keywords
Eucalyptus, plant growth regulators, rooting

 
 fr
Wachira, F.

Résumé

La reproduction des pousses in vitro etait induite des zones orthotropiques du bourgeon de la pousse en etat de recroissance d'un eucalyptus taille et age 25 ans, en utilisant une regeneration d'un media supplemente avec plusieurs differentes combinaisons regulatrices de croissance des plantes, des sources carboniques differentes, et un PH different au moment de l'inoculation. La regeneration optimale de la pousse etait obtenue dans le media de murashige et Skoog supplee avec 0.4 mg l-1 IBA ou 0.2 mg l-1 IBA ou 0.2 mg l-1 BAP et 1.0 mg l-1 NAA et 3% (W/V) du sucrose. Une periode sous-culturelle de 8 semaines etait jugee convenable. NAA et IBA reduisaient la duree de vie de culture des pousses de E. grandis en encourageant la croissance et la proliferation des calles. Le sucrose administre 30 g/l-1 a donne une croissance elevee par rapport aux autres sources carboniques testees, telles que le maltose, le glucose et le fructose. Le PH du media a affecte la croissance des cultures de pousses avec de proportions de 5.8 de PH, assurant par consequent la meilleure croissance.

Mots Clés
Eucalyptus, regulateurs de croissance, encracinement

 
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