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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 3, 2001, pp. 160-168
Bioline Code: ej01029
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 3, 2001, pp. 160-168

 en Trichoderma aureoviride check for this species in other resources 7-121, a mutant with enhanced production of lytic enzymes: its potential use in waste cellulose degradation and/or biocontrol
Zaldívar, Mercedes; Velásquez, Juan Carlos; Contreras, Inés & Pérez, Luz María

Abstract

A mutant of the native fungus Trichoderma aureoviride check for this species in other resources , 7-121, selected for its overproduction of extracellular cellulase and β-glucosidase (cellobiase) was obtained. In shake flask cultures, production of endoglucanase, filter paper activity and cellobiase increased two to four- fold as compared with the wild type strain. The mutant strain is stable and grows rapidly in liquid as well as in solid culture media. Enzyme yields were best when pH was controlled so that it did not fall bellow pH 3.5. Cellobiase production by this mutant is particularly high (approximately 5 U/ml) as compared to other Trichoderma check for this species in other resources , strains, which makes it a suitable candidate for waste cellulose degradation. In addition, the mutant strain showed enhanced production of fungal cell wall degrading enzymes: chitinases, β-1,3-glucanases and proteases. This improvement in extracellular enzyme production by the mutant T. aureoviride 7-121 suggests that it is a suitable strain to be used in biological control.

Keywords
biological control, lytic enzymes, Trichoderma aureoviride check for this species in other resources

 
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