Agrobacterium tumefaciens check for this species in other resources , Penicillium chrysogenum check for this species in other resources , Ti plasmid, transformation"/>
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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 1, 2002, pp. 29-41
Bioline Code: ej02010
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 1, 2002, pp. 29-41

 en Efficient transformation of Penicillium chrysogenum check for this species in other resources mediated by Agrobacterium tumefaciens check for this species in other resources LBA4404 for cloning of Vitreoscilla check for this species in other resources hemoglobin gene
Sun, Chuan-Bao; Kong, Qiu-Lian & Xu, Wen-Si

Abstract

The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum check for this species in other resources under help of T-DNA of Agrobacterium tumefaciens check for this species in other resources LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts.

Keywords
Agrobacterium tumefaciens check for this species in other resources , Penicillium chrysogenum check for this species in other resources , Ti plasmid, transformation

 
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