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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 2, 2002, pp. 141-153
Bioline Code: ej02025
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 2, 2002, pp. 141-153

 en Transfer and expression of the genes of Bacillus check for this species in other resources branched chain alpha-oxo acid decarboxylase in Lycopersicun esculentum check for this species in other resources
Wang-Pruski, Gefu & Szalay, Aladar A.

Abstract

Engineering of higher plants for increased cold tolerance requires a chemical modification of membrane fluidity in both organelles and cytoplasm of plant cells. A small number of microorganisms use branched chain fatty acids as their membrane constituents to maintain membrane fluidity, instead of unsaturated fatty acids. One of the key enzymes facilitating synthesis of branched chain fatty acids in Bacillus subtilis check for this species in other resources 168s is the branched chain alpha-oxo acid decarboxylase. To examine the role of branched chain fatty acids in plants and the potential for low temperature tolerance, the A and B genes encoding the alpha and beta polypeptides, respectively, of the branched chain alpha-oxo acid decarboxylase were introduced into the genome of tomato plants. The mas promoters in the plant expression vector system facilitated the expression of the A and B genes. Transgenic plants were regenerated and shown to produce both the alpha and beta polypeptides. Comparative analysis of a small number of transgenic tomato plants and non-transformed control plants grown at 4ºC showed enhanced cold tolerance in the transformed plants. These findings, if confirmed by a larger scale analysis, suggest a potential role for branched chain fatty acids as a protective mechanism for growth of plants under sub optimal temperatures.

Keywords
branched chain alpha-oxo acid decarboxylase, cold tolerance, gene expression, transgenic tomato plants.

 
© 2002 by Universidad Católica de Valparaíso -- Chile
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