A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 μmol. The developed system was applied using
Tsukamurella paurometabola
DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 μmol in 24 hrs.