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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 6, No. 3, 2003, pp. 223-232
Bioline Code: ej03026
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 6, No. 3, 2003, pp. 223-232

 en Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
García, Armando Hernández; Vázquez, Eulogio Pimentel & Campos, Jesús Mena

Abstract

A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 μmol. The developed system was applied using Tsukamurella paurometabola check for this species in other resources DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 μmol in 24 hrs.

Keywords
bacteria, bacterial growth, conductimetric titration, microorganisms, sulfur-containing gases

 
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