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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 7, No. 2, 2004, pp. 115-123
Bioline Code: ej04013
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 7, No. 2, 2004, pp. 115-123

 en Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus check for this species in other resources levansucrase produced in yeast
Toledo, Luis Enrique Trujillo; Riera, Raúl Gómez; Castillo, Alexander Banguela; Romero, Melvis Soto; Sosa, Juan Gabriel Arrieta & García, Lázaro Hernández

Abstract

The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris check for this species in other resources was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60°C, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures.

Keywords
catalytical properties, fructo-oligosaccharides, fructosyltransferase, levansucrase, N-glycosylation, Pichia pastoris

 
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