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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 7, No. 2, 2004, pp. 210-213
Bioline Code: ej04021
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 7, No. 2, 2004, pp. 210-213

 en Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression
Song, Ping & Wang, Hwa-Chain Robert


The technology of tetracycline-inducible gene expression has been successfully used in experimental biology to identify the function and downstream signaling pathways of an interested gene. It has been significantly improved to meet the criteria with specificity to exogenous non-toxic inducers, independent regulation from cellular pathways, and dose-dependent inducibility and reversibility. However, to establish tetracycline-inducible gene expression in mammalian cells is still a time-and effort-consuming process. With a tetracycline-inducible gene expression system T-REx, we have developed a practical protocol to use the oncogenic H-ras gene as a dominant reporter gene to increase efficiency in attaining desired cell lines in which ectopic expression of a particular gene in cells can be introduced and reversibly induced by the presence or absence of tetracycline in cultures.

10T1/2 cells, Ras, Tetracycline-inducible expression system, tetR, T-REx

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