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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 7, No. 3, 2004, pp. 298-304
Bioline Code: ej04034
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 7, No. 3, 2004, pp. 298-304

 en Recombinant expression of marine shrimp lysozyme in Escherichia coli check for this species in other resources
de-la-Re-Vega, Enrique; García-Orozco, Karina D.; Calderón-Arredondo, Sergio A.; Romo-Figueroa, María Gabriela; Islas-Osuna, María A.; Yepiz-Plascencia, Gloria M. & Sotelo-Mundo, Rogerio R.


Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp ( Penaeus vannamei check for this species in other resources ) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10%. Details of the protein recombinant expression techniques applied to this shrimp protein are presented.

crustacea, lysozyme, penaeideae, Penaeus vannamei, recombinant expression, refolding, shrimp

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