Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 9, No. 5, 2006, pp. 572-579
Bioline Code: ej06077
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 572-579
© Copyright 2006 - Pontificia Universidad Católica de Valparaíso -- Chile
Multiple gene knock-down by a single lentiviral vector expressing an array of short hairpin RNAs|
Stove, Veronique; Smits, Kaatje; Naessens, Evelien; Plum, Jean & Verhasselt, Bruno
RNA interference (RNAi), mediated by short double-stranded RNAs, is a powerful mechanism for posttranscriptional gene silencing. Sustained expression of short hairpin RNA (shRNA) can be accomplished in mammalian cells by viral delivery systems. Using lentiviral constructs, stable gene silencing is established both in dividing and non-dividing cells. Targeting one single gene can lead to the development of escape mutants or may be insufficient to silence redundant pathways. Therefore, simultaneous targeting of multiple genes may be necessary. We have generated a lentiviral vector-based system for expression of multiple shRNAs from a single viral vector, which also encodes an EGFP reporter protein. We show that knock-down of each single gene from multiple target vectors is achieved at an efficiency comparable to that obtained after transduction using single target viral vectors. In this way, we were able to knock-down several members of the human Rho-family GTPases in T cells. Double and triple knock-down persisted after multiple passages of the cells. The ability to inhibit two or more genes simultaneously from one single expression vector further widens the application spectrum of RNAi, both in functional studies and therapeutic strategies.
EGFP, lentiviral gene transfer, multiple knock-down, RNAi, Rho GTPases, shRNA.
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