Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 10, No. 2, 2007, pp. 328 - 335
Bioline Code: ej07031
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 10, No. 2, 2007, pp. 328 - 335
© Copyright 2007 - Pontificia Universidad Católica de Valparaíso -- Chile
TECHNICAL NOTE - Single embryoid body formation in a multi-well plate|
Ezekiel, Uthayashanker R.; Muthuchamyo, Mariappan; Ryerse, Jan S. & Heuertz, Rita M.
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocols.
differentiation, EB, ES, murine embryonic stem cell.
Alternative site location: http://www.ejbiotechnology.info