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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 10, No. 2, 2007, pp. 328 - 335
Bioline Code: ej07031
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 10, No. 2, 2007, pp. 328 - 335

 en TECHNICAL NOTE - Single embryoid body formation in a multi-well plate
Ezekiel, Uthayashanker R.; Muthuchamyo, Mariappan; Ryerse, Jan S. & Heuertz, Rita M.

Abstract

Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocols.

Keywords
differentiation, EB, ES, murine embryonic stem cell.

 
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