Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 11, No. 3, 2008
Bioline Code: ej08032
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 11, No. 3, 2008
© Copyright 2008 - Pontificia Universidad Católica de Valparaíso -- Chile
Development of transformation vectors for the production of potentially high oleate transgenic oil palm|
Yunus, A.M.M. & Parveez, G.K.A.
The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense D9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.
oleate, transformation vectors, transgenic oil palm.
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