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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 13, No. 4, 2010
Bioline Code: ej10037
Full paper language: English
Document type: Report
Document available free of charge

Electronic Journal of Biotechnology, Vol. 13, No. 4, 2010

 en Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga check for this species in other resources through tissue culture
Parida, Reena; Mohanty, Sujata; Kuanar, Ananya & Nayak, Sanghamitra

Abstract

An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga check for this species in other resources L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of leaves after 2 months of culture under sterile condition, the base of each plantlet was transferred to fresh media which could produce the same leaf biomass within another 2 months in a 50 ml culture tube containing 20 ml and 250 ml conical flasks containing 30 ml Murashige and Skoog medium. The rate of multiplication and leaf biomass production remained unchanged in subsequent subcultures. The regenerated plantlets were acclimatized in greenhouse and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 95 percent. Genetic fidelity of the regenerants was confirmed using random amplified polymorphic DNA (RAPD) marker. The protocol could be commercially utilized for large scale production of true-to-type plantlets and as an alternative method of leaf biomass production in Kaempferia galanga.

Keywords
growth regulators, Kaempferia, in vitro propagation, leaf biomass.

 
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