Background: Optimization of nutrient feeding was developed to improve the growth of
Bacillus subtilis
in fed
batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was
used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry
weight in the culture broth with fed batch fermentation in a 5 L fermentor.
Results: The results were analyzed using response surface methodology (RSM), and the optimized values of
glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest
cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch
fermentation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation
as compared to the production of batch fermentation.
Conclusion: The results of this work improve the present production of JAA and may be adopted for other
objective products' production.
