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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 18, No. 3, 2015, pp. 244-251
Bioline Code: ej15040
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 18, No. 3, 2015, pp. 244-251

 en Enhanced production of dimethyl phthalate-degrading strain Bacillus check for this species in other resources sp. QD14 by optimizing fermentation medium
Mo, Jixian; Wang, Zhigang; Xu, Weihui; Li, Shanshan & Yu, Zhidan

Abstract

Background: Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett–Burman design (PBD) was applied to screen for significant factors, followed by the Steepest Ascent Method (SAM) to find the nearest region of maximum response. A Box–Behnken design (BBD) of the Response Surface Methodology (RSM) was conducted to optimize the final levels of the medium components.
Results: After the regression equation and response surface contour plots were analyzed, the concentrations of glucose, corn meal and NaCl were found to significantly influence the biomass of DMP-degrading bacteria. A combination of 22.88 g/L of glucose, 11.74 g/L of corn meal, and 10.34 g/L of NaCl was optimum for maximum biomass production of Bacillus sp. QD14. A 57.11% enhancement of the biomass production was gained after optimization in shake-flask cultivation. The biomass production of Bacillus sp. QD14 reached 9.13 ± 0.29 × 108 CFU/mL, which was an excellent match for the predicted value, and the mean value of the match degree was as high as 99.30%.
Conclusion: In this work, the key factors affected by the fermentation of DMP-degrading strain Bacillus sp. QD14 were optimized by PBD, SAM and BBD (RSM); the yield was increased by 57,11% in the conditions in our study. We propose that the conditions optimized in the study can be applied to the fermentation for commercialization production.

Keywords
Bacillus; Box–Behnken design; Dimethyl phthalate; Medium optimization; Plackett–Burman design

 
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