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The Journal of Food Technology in Africa
Innovative Institutional Communications
ISSN: 1028-6098
Vol. 7, No. 3, 2002, pp. 93-97
Bioline Code: ft02022
Full paper language: English
Document type: Research Article
Document available free of charge

The Journal of Food Technology in Africa, Vol. 7, No. 3, 2002, pp. 93-97

 en Identification of Lactic Acid Bacteria isolated from Opaque beer (Chibuku) for potential use as a starter culture
Chamunorwa A.Togo, Sara B. Feresu, Anthony N. Mutukumira

Abstract

A study was carried out to identify lactic acid bacteria (LAB) isolated from chibuku that would be later assessed for potential as starter cultures. Thirty-eight isolates were Gram stained and the 20, which were Gram positive, were identified to genus level using morphological, physiological and biochemical tests. Five genera of lactic acid bacteria were identified and these were Lactobacillus check for this species in other resources (seven isolates coded B1, B2, C4, E3, E6, F1 and F4), Lactococcus check for this species in other resources (five isolates: E1, F5, G5, G6 and H1), Leuconostoc check for this species in other resources (three isolates: E2, D5, F6), Streptococcus check for this species in other resources (two isolates: G2 and G4) and Enterococcus check for this species in other resources (three isolates: B3, B4 and G3).

From these genera, eleven isolates five from the genus Lactobacillus, three from Lactococcus and three from Leuconostoc were selected for identification to species level using API 50 CH kits. The Lactobacillus strains were identified as follows: two strains were Lb plantarum (C4 and F4), two strains Lb. delbrueckii (B2 and E3), one strain could not be assigned to a species and was termed Lactobacillus sp. E6. Two of the Lactococcus isolates were identified as Lc. lactis subsp. lactis (G6 and H1), while the third isolate was Lc. raffinolactis (strain F5). The three Leuconostoc strains were Ln. mesenteroides subsp. mesenteroides.

Keywords
Lactic acid bacteria; Sorghum beer (chibuku check for this species in other resources ); Starter culture; Identification

 
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