Radiation-induced chromosomal hot spots at G1 and G2 stages of human lymphocytes in culture

Radiation-induced chromosomal break points in cultured lymphocytes of normal healthy individuals as well as of those with certain genetic disorders are reported to be localized at certain specific loci (hot spots). These reports are based on studies carried out in lymphocytes irradiated at G₁ stage. The present study examines whether the location of hot spots and the frequency seen in cells irradiated at G₁ are similar to those irradiated at G₂ stage of the cell cycle and also tests whether cells of patients exhibit hot spots on irradiation.The results showed that the radiation induced chromosomal break points to be similar in those irradiated are G₁ and G₂ stages of the cell cycle and also that cells of patients exhibited chromosomal hot spots.