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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 9, No. 4, 2009, pp. 212-217
Bioline Code: hs09055
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 9, No. 4, 2009, pp. 212-217

 en Characterization of plasma membrane bound inorganic pyrophosphatase from Leishmania donovani check for this species in other resources promastigotes and amastigotes
Sen, SS; Bhuyan, NR & Bera, T


Background: Currently, a major problem in the management of visceral leishmaniasis or kala-azar, especially in the Indian subcontinent, is the growing unresponsiveness to conventional antimonial therapy. Membrane bound pyrophophatase (PPases) do not exist in plasma membrane from mammals. Thus, H+-PPases from Leishmania check for this species in other resources plasma membrane might be potential target in rational chemotherapy of the disease caused by Leishmania parasites.
Objective: To characterize the activities of inorganic pyrophophatase (PPase) in the plasma membrane of Leishmania donovani check for this species in other resources promastigote and amastigote.
Methods: Culture method of promastigote and amastigote were developed. We assayed PPase activity in isolated plasma membrane of L. donovani.
Results: We characterized K+-PPase present in the plasma membrane of Leishmania donovani and investigated its possible role in the survival of promastigote and amastigote. PPase activity was stimulated by K+ ions and sodium orthovanadate, inhibited by pyrophosphate analogs imidodiphosphate and alendronate, KF, DCCD, thiol reagent parachloromercuribenzenesulfonate (PCMBS), N-ethylmaliemide (NEM), phenylarsineoxide, ABC superfamily transport modulator verapamil and was also by F1Fo-ATPase inhibitor quercetin.
Conclusion: We conclude that there are significant differences within promastigote, amastigote and mammalian host in cytosolic pH homeostasis to merit the inclusion of PPase transporter as putative targets for rational drug design.

Leishmania donovani; pyrophosphatase; plasma membrane

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