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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 11, No. 4, 2007, pp. 95-100
Bioline Code: ja07103
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 11, No. 4, 2007, pp. 95-100

 en Kinetic And Thermodynamic Studies Of Glucose Oxidase Catalysed Oxidation Reaction of Glucose
Odebunmi, E.O. & Owalude, S.O.

Abstract

The kinetics of oxidation of D-glucose catalysed by the enzyme glucose oxidase has been studied over a wide range of experimental conditions. The reaction velocities increased with increase in the concentrations of the glucose oxidase and glucose, as well as increase in temperature and ionic strength of the solution. The reaction velocity initially increased with increase in pH, reaching a maximum at pH 6.5 and then decreased with further increase in pH. The reaction exhibited saturation kinetics and experimental data were analysed using the Michaelis- Menten equation. Arrhenius activation energy and thermodynamic activation parameters were measured and are reported. The large negative value of entropy of activation ΔS , -148.8JK-1mol-1, and positive value of the enthalpy of activation ΔH , 26.3kJmol-1, give further support to the proposed mechanism. The results are interpreted in terms of a mechanism involving both an oxidative half reaction and a reductive half reaction.

 
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