Prompt detection of metallo-β-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR)
Acinetobacter
species and
Pseudomonas aeruginosa
was evaluated in critical care patients using imipenem-EDTA disk method. One hundred MDR
Acinetobacter spp. and 42
Pseudomonas aeruginosa were checked for MBL production, from January to June 2001. MBL was produced by 96.6 % of imipenem-resistant
Acinetobacter isolates, whereas 100% imipenem-resistant
Pseudomonas aeroginosa isolates were MBL producers. Carbapenem resistance in MDR
Acinetobacter spp. and
Pseudomonas aeruginosa isolates in this study was due to MBLs. This calls for strict infection control measures to prevent further dissemination.