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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 27, No. 2, 2009, pp. 128-133
Bioline Code: mb09039
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 27, No. 2, 2009, pp. 128-133

 en Identification of Lysine Positive Non-fermenting Gram Negative Bacilli ( Stenotrophomonas maltophilia check for this species in other resources and Burkholderia cepacia check for this species in other resources Complex)
Gautam, V.; Ray, P.; Vandamme, P.; Chatterjee, S.S.; Das, A.; Sharma, K.; Rana, S.; Garg, R.K.; Madhup, S.K.; Mahajan, M. & Sharma, M.

Abstract

Background: The Burkholderia cepacia check for this species in other resources complex (BCC) and Stenotrophomonas maltophilia check for this species in other resources are closely related groups of non-fermenting gram-negative bacilli (NFGNBs) having a similar spectrum of infections ranging from superficial to deep-seated and disseminated infections. Identification of these lysine decarboxylase-positive NFGNBs lags behind in most Indian laboratories. A simplified identification scheme was devised for these two pathogens that allowed us to isolate them with an increasing frequency at our tertiary care institute.
Materials and Methods: A simple five-tube conventional biochemical identification of these bacteria has been standardized. In the beginning, some of the isolates were confirmed from the International B. cepacia Working group, Belgium. Molecular identification and typing using recA polymerase chain reaction-restriction fragment length polymorphism was also standardized for BCC. For short-term preservation of BCC, an innovative method of preserving the bacteria in Robertson′s cooked medium tubes kept in a domestic refrigerator was developed.
Results: Thirty-nine isolates of BCC isolates were obtained from various specimens (30 from blood cultures) and 22 S. maltophilia (13 blood cultures and 9 respiratory isolates) were isolated during the year 2007 alone.
Conclusions: BCC and S. maltophilia can be identified with relative ease using a small battery of biochemical reactions. Use of simplified methods will allow greater recognition of their pathogenic potential and correct antimicrobials should be advised in other clinical laboratories and hospitals.

Keywords
Burkholderia cepacia complex, identification, India, stenotrophomonas maltophilia

 
© Copyright 2009 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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