Opportunistic infections caused by Non-
Candida albicans
have been increasing. Traditional methods that are used to identify clinical isolates of
Candida species are time-consuming and not appropriate for rapid, accurate and
reliable identification.
Purpose: To identify
Candida spp isolated from cancer patients using PCR-restriction enzyme.
Materials and methods:
Using universal primers, ITS1 and ITS4, in this study, we could amplify ITS1-5.8S-ITS2 rDNA regions at both 80 clinical isolates and 3 standard strains. The PCR products were digested
with two restriction enzymes M
spI and B
lnI separately.
Result: We successfully identified all isolated species using two restriction enzymes (M
spI
,
B
lnI).
Candida albicans was the most common species (77.5%), followed by
C. glabrata (15%),
C. tropicalis (5%),
C.
krusei (2.5%). Although the primers and enzyme had the ability to identify
C.
parapsilosis,
C.
guilliermondii,
C.
dubliniensis, present isolates did not include these among identified ones.
Conclusion: RFLP-PCR using ITSI and ITS4 primers and restriction enzyme is a rapid, easy, reliable and also applicable method in clinical laboratory for identification of medically important
Candida spp.