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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857 EISSN: 0255-0857
Vol. 28, No. 4, 2010, pp. 290-294
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Bioline Code: mb10092
Full paper language: English
Document type: Research Article
Document available free of charge
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Indian Journal of Medical Microbiology, Vol. 28, No. 4, 2010, pp. 290-294
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Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing
Mehta, P. R.; Nema, S.; Paranjpe, S.; Ingole, N.; Wanjare, S. & Nataraj, G.
Abstract
Aims: To determine the prevalent subtypes of HIV-1 in serodiscordant couples.
Setting: Integrated Counselling and Testing Centre (ICTC), Department of Microbiology.
Study Design: Prospective pilot study.
Participants: Thirty HIV-1 serodiscordant couples.
Inclusion Criteria: a) Documentation of HIV-1 infection in one partner and seronegative status in the other, current history of continued unprotected sexual activity within the partnership, demonstration that they have been in a partnership for at least 1 year and are not currently on highly active antiretroviral therapy HAART; b) willingness of both partners to provide written informed consent including consent to continued couple counselling for 3 months.
Materials and Methods: HIV-1 subtyping was carried out by heteroduplex mobility analysis (HMA) by amplifying env region; and DNA sequencing by amplifying gag region.
Results: HIV-1 env gene was amplified successfully in 10/30 samples; gag gene, in 25/30 samples; and both env and gag gene were amplified successfully in 5/30 samples. HIV-1 subtype C was detected from 21 samples; subtype B, from 7; and subtype A, from 2. Sample from 1 positive partner was detected as subtype C by env HMA and subtype B by gag sequencing.
Conclusion: HIV-1 subtype C was found to be the predominant subtype of HIV-1 in serodiscordant couples attending our ICTC, followed by HIV-1 subtype B and HIV-1 subtype A, respectively. DNA sequencing was found to be the most reliable method for determining the subtypes of HIV-1.
Keywords
DNA sequencing, HIV-1 subtypes, heteroduplex mobility analysis, serodiscordant couples
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© Copyright 2010 Indian Journal of Medical Microbiology. Alternative site location: http://www.ijmm.org
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