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Indian Journal of Medical Microbiology
Medknow Publications on behalf of Indian Association of Medical Microbiology
ISSN: 0255-0857
EISSN: 0255-0857
Vol. 30, No. 1, 2012, pp. 34-38
Bioline Code: mb12007
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Medical Microbiology, Vol. 30, No. 1, 2012, pp. 34-38

 en In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin against Pseudomonas aeruginosa check for this species in other resources
Bozkurt-Guzel, C & Gerceker, A A.

Abstract

Purpose: In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin were investigated by studying time-kill kinetics and post-antibiotic effect (PAE) against strains of Pseudomonas aeruginosa check for this species in other resources isolated from patients with cystic fibrosis.
Method: Synergy was investigated at 0.5×, 1× and 5× MIC of antibiotics using time-kill curve method. PAEs were determined by the standard viable counting method where bacteria in the logarithmic phase of growth were exposed for 1 h to the antibiotics at 1× or 20× MIC, alone and in combinations. Synergy and additive effects were detected at 1×MIC, at 24 h.
Results: Some of the strains produced an earlier synergistic effect at 12 h. No antagonism was observed. Colistin methanesulfonate and amikacin produced PAEs 1.16 ± 0.10 to 2.25 ± 0.16 h and 0.96 ± 0.15 to 2.69±0.32 h, respectively. When the antibiotics were used in combination the PAEs were prolonged to a value of 3.88±0.25 h. Consequently, the
Conclusions: Findings of this study may play useful role in selecting the appropriate combinations when a single agent is inadequate, and may have important information for optimizing the dose intervals.

Keywords
Antibiotic combinations, colistin methanesulfonate, cystic fibrosis, Pseudomonas aeruginosa, postantibiotic effect

 
© Copyright 2012 Indian Journal of Medical Microbiology.
Alternative site location: http://www.ijmm.org

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