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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 9, No. 1, 2006, pp. 57-62
Bioline Code: md06009
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Biomedical Research, Vol. 9, No. 1, 2006, pp. 57-62

 en Bioautographic Evaluation of Antibacterial Metabolite Production by Wild Mushrooms
Shittu, O.B. Alofe, F.V. Onawunmi, G.O. Ogundaini, A.O. and Tiwalade, T.A

Abstract

Russula sp. and Pycnoporus cinnabarinus check for this species in other resources were subjected to liquid and solid state fermentation for metabolite production. Local rice substrate was used for the solid state fermentation to provide a cheap and readily available medium for laboratory cultivation of wild mushroom. Bioautography, a technique that combines chromatography with bioassay in situ allows the localization of the active constituent. The result showed that in submerged fermentation, both the culture filterate and mycelial extract of Russula sp. displayed antimicrobial activity while only the culture filtrate demonstrated activity in Pycnoporus cinnabarinus.. The activity of the solid state fermentation was exhibited by the ethyl acetate fractions while the aqueous fraction showed no activity. The comparison of the culture filtrates (ethyl acetate fractions) of submerged fermentation and the ethyl acetate fractions of solid state fermentation displayed similar activities.

Keywords
Bioautography, Mushroom, Metabolite, Solid state, Fermentation.

 
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