Pharmacological effects of low- dose of aspirin on ovulation rate in mature cycling female mice

Objective: To determine the effects of low-dose of aspirin on ovarian response, uterine and ovarian blood flow supply, and ovulation rate in mature cycling female mice.
Design: Mature cycling female mice treated with aspirin on the first day of proestrous phase.
Setting: Institute of the Embryo Research and Infertility Treatment- University of Baghdad.
Materials and methods: Mature cycling female mice treated subcutaneously twice/day with Aspirin (7.5mg/Kg.b.w)on the first day of proestrous phase. In the treated six groups (6 mice/ group) underwent subcutaneous administration of aspirin at a dose level of (7.5mg/Kg b.w) twice daily at proestrous phase of the estrous cycle. In the control six group (6mice/ group) underwent subcutaneous administration of a placebo (distilled water) twice daily at proestrous phase of the estrous cycle Main outcome measure(s): Uterine and ovarian morphological changes, uterine and ovarian weight changes, number of ova and serum (FSH, LH and E2) levels.
Results: There were statistically significant differences between the treated group and the control group, respectively, in the Ovarian weight (3.14 ± 0.03 versus 2.58 ± 0.06), number of ova (6.00 ± 0.37 versus 11.17 ± 0.40). Conclusion(s): Short-term administration of a low-dose of aspirin to mice at proestrous causes the following changes: significant increase in ovarian weight with an increased congestion in the uterus and ovary. A significant decrease in ovulatory rate with no effect on serum gonadotropins and estradiol levels.

Keywords
Low-dose aspirin, uterine and ovarian morphological changes, and ovulation rate.