Malaysian Journal of Medical Sciences
School of Medical Sciences, Universiti Sains Malaysia
Vol. 24, No. 6, 2017, pp. 50-57
Bioline Code: mj17078
Full paper language: English
Document type: Research Article
Document available free of charge
Malaysian Journal of Medical Sciences, Vol. 24, No. 6, 2017, pp. 50-57
© Copyright 2017 - Penerbit Universiti Sains Malaysia
Low-dose Nicotine Exposure Induced the Oxidative Damage of Reproductive Organs and Altered the Sperm Characteristics of Adolescent Male Rats|
Budin, Siti Balkis; Kho, Jia Hui; Lee, Jia Hui; Ramalingam, Anand; Fatin Farhana, Jubaidi; Elda Surhaida, Latif; Satirah, Zainalabidin; Izatus Shima, Taib & Jamaludin, Mohamed
Background: Nicotine is a major toxic and hazardous component of cigarette smoke, and
it has been widely used in nicotine replacement therapy (NRT). This study was aimed to investigate
the effects of chronic low-dose nicotine on sperm characteristics and reproductive organ integrity
in adolescent male Sprague–Dawley rats.
Methods: Twelve rats were equally divided into two groups. Group I received normal
saline, and group II received 0.6 mg/kg body weight nicotine intraperitoneally for 28 consecutive
days. At the end of the experimental period, sperm was collected for sperm characteristic
evaluation, and the testes and prostate were isolated for biochemical and morphological analysis.
The effects of nicotine on the body and reproductive organ weights of the animals were evaluated.
Results: Chronic nicotine treatment significantly (P < 0.05) altered the sperm count,
motility, viability, and morphology, and remarkably increased the malondialdehyde (P < 0.001)
and advanced oxidation protein product (P < 0.05) levels in the testes and prostate of nicotinetreated
group compared to control group. Moreover, nicotine caused a significant decrease
(P < 0.05) in the superoxide dismutase activity of the testes. No significant differences were
observed in the reduced glutathione level in both of the testes and prostate of nicotine group
compared with control group. Nicotine also induced histopathological alteration in the testes.
Conclusion: A low-dose nicotine exposure at 0.6 mg/kg caused detrimental effects on
sperm characteristics and induced oxidative stress in the testes and prostate.
infertility; prostate; testes; sperm; male; reproductive
Alternative site location: http://www.medic.usm.my/publication/mjms/