search
for
 About Bioline  All Journals  Testimonials  Membership  News


Medical Journal of The Islamic Republic of Iran
National Research Centre of Medical Sciences of I.R. IRAN
ISSN: 1016-1430
Vol. 19, No. 2, 2005, pp. 181-184
Bioline Code: mr05015
Full paper language: English
Document type: Research Article
Document available free of charge

Medical Journal of The Islamic Republic of Iran, Vol. 19, No. 2, 2005, pp. 181-184

 en DETECTION OF HAEMOPHILUS INFLUENZAE check for this species in other resources TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BY PCR
F.A. NAKHJAVANI, F. BONAKDAR HASHEMI, M.T.KALANI,B. KAZEMI, K. NOURI, N.AZADI, M. HAGHI ASHTIANI, M. ALIGHOLI, U. ERFANI, AND M. ABEDINI

Abstract

Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae check for this species in other resources type b (Hib) in CSF samples from children suspected of meningitis.
Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR.
Results: The latter method could detect Hib in all 5 culture positive and in 2 of 295 culture negative cases, showing sensitivity, specificity, and an accuracy index of 100%, 99% and 99%, respectively. The PCR result was available within a day.
Conclusion: This study has shown that PCR is a rapid, sensitive, and specific diagnostic test for Hib from CSF samples. Furthermore, to maximize management of meningitis cases to reduce the morbidity, mortality, and complications of invasive Hib infection a combination of culture and PCR is necessary for the detection of Hib infection. The incidence of Hib meningitis among children in Children's Medical Center in Tehran based on culture and PCR results were 2.4 % and 1.7% respectively. Based on culture results, Hib causes 15.6% of all cases of bacterial meningitis in children.

Keywords
Haemophilus influenzae type b; Meningitis; PCR; culture; CSF; Iran; Incidence.

 
© Copyright 2005 -Medical Journal of the Islamic Republic of Iran
Alternative site location: http://mjiri.iums.ac.ir/index.php/mjiri

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil