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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 171-177
Bioline Code: oc00030
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 171-177

 en Identification of Planorbids from Venezuela by Polymerase Chain Reaction Amplification and Restriction Fragment Length Polymorphism of Internal Transcriber Spacer of the RNA Ribosomal Gene
Roberta L Caldeira; Teofânia HDA Vidigal; Liboria Matinella; Andrew JG Simpson & Omar S Carvalho

Abstract

Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Morphological identification was carried out by comparison of characters of the shell and the male and female reproductive apparatus. The PCR-RFLP involved amplification of the internal spacer region ITS1 and ITS2 of the RNA ribosomal gene and subsequent digestion of this fragment by the restriction enzymes DdeI, MnlI, HaeIII and MspI. The planorbids were compared with snails of the same species and others reported from Venezuela and present in Brazil, Cuba and Mexico. All the enzymes showed a specific profile for each species, that of DdeI being the clearest. The snails were identified as B. glabrata, B. prona and B. kuhniana.

Keywords
Biomphalaria glabrata - Biomphalaria kuhniana - Biomphalaria prona - snails - internal transcribed spacer - ribosomal DNA

 
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