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Isotype Specific Immune Responses in Murine Experimental Toxocariasis
C Cuéllar; S Fenoy; C del Águila & JL Guillén
Abstract
In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and
C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate
the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific
IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested by ELISA. The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely
to the results obtained with the IgM isotype, the IgG antibody class showed similar patterns to those obtained with IgG+IgM antibodies, only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+IgM antibodies, when the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conversely, in the C3H strain only IgG
specific antibody levels were detected. The IgG1 subclass responses showed a similar profile in the three
murine strains studied, with high values in BALB/c, as in the case of the IgG responses.
Keywords
Toxocara canis, BALB/c, C57BL/10, C3H, ELISA, immunoglobulins
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