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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. s1, 2001, pp. 79-83
Bioline Code: oc01155
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. s1, 2001, pp. 79-83

 en Preliminary Analysis of Sm14 in Distinct Fractions of Schistosoma mansoni check for this species in other resources Adult Worm Extract
Nilton Thaumaturgo; Mônica Magno Vilar; Catia Maria Diogo; Ricardo Edelenyi & Miriam Tendler

Abstract

In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni check for this species in other resources infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica check for this species in other resources in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE, and is being developed as the molecular basis of an anti-helminth bivalent vaccine against the two parasites, for medical and veterinary application. Present data refer to SDS-PAGE and Western Blotting analysis of four different preparations of S. mansoni check for this species in other resources adult worms focusing Sm14 identification. The extracts correspond to the initial fraction of the SE extraction process, containing products released by living worms (SEi); SE2, reextraction of adult worms in PBS; and SE of separated male and female adult worms. In all extracts it was possible to detect the component of 14 kDa, that was recognized by specific anti-rSm14 antibody raised in rabbits.

Keywords
Schistosoma mansoni, vaccine, excretory/secretory antigens, FABP, Sm14

 
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