Trypanosoma cruzi check for this species in other resources - Calomys callosus check for this species in other resources - myocardial fibrosis - collagen immunotyping - interstitial matrix"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 5, 2002, pp. 703-710
Bioline Code: oc02135
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 5, 2002, pp. 703-710

 en Fibrogenesis and Collagen Resorption in the Heart and Skeletal Muscle of Calomys callosus check for this species in other resources Experimentally Infected with Trypanosoma cruzi check for this species in other resources : Immunohistochemical Identification of Extracellular Matrix Components
Isis F Magalhães-Santos; Elianita S Lima & Sonia G Andrade

Abstract

Intense inflammatory lesions and early development of interstitial fibrosis of the myocardium and skeletal muscle with spontaneous regression, have been described in Calomys callosus check for this species in other resources infected with Trypanosoma cruzi check for this species in other resources . The genetic types of collagen present in this model were investigated through immunohistochemistry using specific antibodies, combined with histopathology and Picro-Sirius staining of collagen.
Thirty-five calomys were infected with the Colombian strain of T. cruzi and sacrificed at 24, 30, 40, 60 and 90 days post-infection. Inflammatory lesions and fibrogenesis were prominent at the early phase of infection and significantly decreased during late infection. Immunoisotyping of the matrix components was performed by indirect immunofluorescence on 5 µm thick cryostat sections using specific antibodies against laminin, fibronectin and isotypes I, III and IV of collagen. In the early phase, positive deposits of all the matrix components were present, with predominance of fibronectin, laminin and collagens types I and III in the myocardium and of types III and IV in the skeletal muscles. From the 40th day, type IV collagen predominates in the heart. At the late phase of infection (60th to 90th day), a clear fragmentation and decrease of all the matrix components were detected. Findings of the present study indicate that a modulation of the inflammatory process occurs in the model of C. callosus , leading to spontaneous regression of fibrosis independent of the genetic types of collagen involved in this process.

Keywords
Trypanosoma cruzi check for this species in other resources - Calomys callosus check for this species in other resources - myocardial fibrosis - collagen immunotyping - interstitial matrix

 
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