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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 47-52
Bioline Code: oc02186
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 47-52

 en Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of Cytocrome Oxidase Subunit I Used for Differentiation of Brazilian Biomphalaria check for this species in other resources Species Intermediate Host of Schistosoma mansoni check for this species in other resources
Teofânia HDA Vidigal; Lângia C Montresor; Andrew JG Simpson & Omar S Carvalho

Abstract

The intermediate hosts of Schistosoma mansoni check for this species in other resources , in Brazil, Biomphalaria glabrata check for this species in other resources , B. tenagophila and B. straminea , were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina.

Keywords
Biomphalaria check for this species in other resources - polymerase chain reaction - restriction fragment length polymorphism - cytochrome oxidase I

 
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