Calomys callosus check for this species in other resources - Rodentia - Cricetidae - Schistosoma mansoni check for this species in other resources - intestine - nodules - fibrosis - angiogenesis"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 117-127
Bioline Code: oc02200
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 117-127

 en Intestinal Fibrovascular Nodules Caused by Schistosoma mansoni check for this species in other resources Infection in Calomys callosus check for this species in other resources Rengger, 1830 (Rodentia: Cricetidae): a Model of Concomitant Fibrosis and Angiogenesis
Jane A Lenzi; Ester M Mota; Marcelo Pelajo-Machado; Leandro S Vale; Bruno S Vale; Zilton A Andrade & Henrique L Lenzi

Abstract

Human schistosomiasis develops extensive and dense fibrosis in portal space, together with congested new blood vessels. This study demonstrates that Calomys callosus check for this species in other resources infected with Schistosoma mansoni check for this species in other resources also develops fibrovascular lesions, which are found in intestinal subserosa. Animals were percutaneously infected with 70 cercariae and necropsied at 42, 45, 55, 80, 90 and 160 days after infection. Intestinal sections were stained for brightfield, polarization microscopy, confocal laser scanning, transmission and scanning electron microscopies. Immunohistological analysis was also performed and some nodules were aseptically collected for cell culture.
Numerous intestinal nodules, appearing from 55 up to 160 days after infection, were localized at the interface between external muscular layer and intestinal serosa, consisting of fibrovascular tissue forming a shell about central granuloma(s). Intranodular new vessels were derived from the vasculature of the external vascular layer and were positive for laminin, chondroitin-sulfate, smooth muscle alpha-actin and FVIII-RA. Fibroblastic cells and extracellular matrix components (collagens I, III and VI, fibronectin and tenascin) comprised the stroma. Intermixed with the fibroblasts and vessels there were variable number of eosinophils, macrophages and haemorrhagic foci.
In conclusion, the nodules constitute an excellent and accessible model to study fibrogenesis and angiogenesis, dependent on S. mansoni eggs. The fibrogenic activity is fibroblastic and not myofibroblastic-dependent. The angiogenesis is so prominent that causes haemorrhagic ascites.

Keywords
Calomys callosus check for this species in other resources - Rodentia - Cricetidae - Schistosoma mansoni check for this species in other resources - intestine - nodules - fibrosis - angiogenesis

 
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