hilA
gene promoter, component of the
Salmonella
Pathogenicity Island
1, has been found in
Salmonella serovar Typhimurium, being important for
the regulation of type III secretion apparatus genes. We detected
hilA
gene sequences in
Salmonella serovars Typhi, Enteritidis, Choleraesuis,
Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization
techniques. The primers to carry out PCR were designed according to
hilA
sequence. A low stringency hybridization with the probe pVV441 (
hilA open-reading-frame
plasmid) was carried out. To find
hilA gene sequences in other
Salmonella
sp. suggest that these serovars could have similar sequences of this kind of virulence
genes.