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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 8, 2002, pp. 1153-1156
Bioline Code: oc02251
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 8, 2002, pp. 1153-1156

 en Detection of hilA Gene Sequences in Serovars of Salmonella enterica subsp. enterica check for this species in other resources
Nora Cardona-Castro; Eliana Restrepo-Pineda & Margarita Correa-Ochoa

Abstract

hilA gene promoter, component of the Salmonella check for this species in other resources Pathogenicity Island 1, has been found in Salmonella serovar Typhimurium, being important for the regulation of type III secretion apparatus genes. We detected hilA gene sequences in Salmonella serovars Typhi, Enteritidis, Choleraesuis, Paratyphi A and B, and Pullorum, by polymerase chain reaction (PCR) and hybridization techniques. The primers to carry out PCR were designed according to hilA sequence. A low stringency hybridization with the probe pVV441 (hilA open-reading-frame plasmid) was carried out. To find hilA gene sequences in other Salmonella sp. suggest that these serovars could have similar sequences of this kind of virulence genes.

Keywords
hil check for this species in other resources A gene sequences - Salmonella check for this species in other resources serovars Typhi - Enteritidis - Choleraesuis - Paratyphi A and B - Pullorum

 
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