Nyssorhynchus check for this species in other resources - Anopheles check for this species in other resources - genetic variability - isozymes - Amazon - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 2, 2003, pp. 247-253
Bioline Code: oc03063
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 2003, pp. 247-253

 en Isoenzymatic Variability among Five Anopheles check for this species in other resources Species Belonging to the Nyssorhynchus check for this species in other resources and Anopheles Subgenera check for this species in other resources of the Amazon Region, Brazil
Joselita Maria Mendes dos Santos; Juracy de Freitas Maia; Wanderli Pedro Tadei & Gloria Alícia Diaz Rodriguez

Abstract

An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles check for this species in other resources species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi , An. albitarsis and An. triannulatus of the Nyssorhynchus check for this species in other resources subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic intere specific divergence. Samples from Macap á , State of Amap á and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi , An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters.

Keywords
Nyssorhynchus check for this species in other resources - Anopheles check for this species in other resources - genetic variability - isozymes - Amazon - Brazil

 
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