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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 3, 2003, pp. 387-390
Bioline Code: oc03087
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 3, 2003, pp. 387-390

 en SHORT COMMUNICATION - A Technique for Preparing Polytene Chromosomes from Aedes aegypti check for this species in other resources (Diptera, Culicinae)
Jairo Campos; Carlos Fernando S Andrade & Shirlei M Recco-Pimentel

Abstract

Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti check for this species in other resources . The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20°C provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the preparation technique of the sheets submitted to a stronger treatment starting with the hypotony of tissue and successive bathings with acetic acid. A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes.

Keywords
cytology - mosquito - vector

 
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